The interaction between osteoblasts and vascular endothelial cells co-culture in vitro

Objective: To explore the interaction between rabbit osteoblasts(ROB) and rabbit vascular endothelial cells(RVEC) co-culture in virto. Methods: Rabit osteoblasts and vascular endothelial cells were isolated and co-cultured in ratios of 1:0, 1:1, 2:1 and 4:1 within culture chambers. The two kinds of cells were also co-cultured in ratios of 0:1, 1:1, 2:1 and 4:1 on coverslip. The metabolic function of osteoblast was assessed by alkaline phosphatase (ALP ) activity and osteocalcin (OC) assay. Morphology of two kinds of cells and proliferation of vascular endothelial cells was observed.Results: The biologic compatibility was good between osteoblasts and vascular endothelial cells. The activity of ALP and OC in group with ratio of 2:1 was higher than the other two groups, but no difference was found compared with control group. The proliferation of RVEC was higher than the other two groups. Conclusions: Synergistic effect was demonstrated by co-culture of osteoblasts and vascular endothelial cells.