The influence of biological characterisitics of osteoblasts cocul tured with endothelial cells in vitro

Objective To explore the influence of bio logical characteristics of osteoblasts cocultured with endothelial cells in vitr o.Methods Human embryonic osteoblasts were cocultured wit h endothelial cells directively or indirectively, osteogenesis were detected by inverted microscope, cell count method, osteocalcin and alkaline phosphatase (AL P) activity assay respectively. Results There were good c ellular compatibility when cocultured of osteoblasts and endothelial cells. The proliferation of osteoblasts were accelerated(P0.05). The ALP activity of d irective or indirective coculture of endothelial cells and osteoblasts were (8.2 00±0.755)μmol/(min·10~6细胞) and (12.300±1.300)μmol/(min·10~6细胞) r espe ctively, which was higher than simple osteoblasts (P0.05). The osteocalcin synthesis of directive or indirective coculture of endothelial cells and osteobl asts were (3.8267±0.4077)μg/L and (5.1533±0.5965)μg/L respective ly,which was higher than simple osteoblasts (P0.05).Conclusion The biological characteristics of osteoblasts can be regulated through cocult ure with endothelial cells in vitro.