Relationship of expression of HPA,HIF-1α in human esophageal squamous cell carcinoma with its invasion and metastasis

Objective: To investigate the relationship of expression of HPA and HIF-1α in human esophageal squamous cell carcinoma with its invasion and metastasis. Methods:The expression of HPA mRNA and HIF-1α protein in 23 normal tissue specimens of incised margin and 70 esophageal squamous cell carcinoma specimens were examined by in situ hybridization assay and immunohistochemical stain respectively. Results:HPA mRNA and HIF-1α protein were positive in 65.7% and 55.7% of all 70 cancer tissue specimens, respectively,which were significantly higher than those in incised margin normal tissues(P0.05). The high expression of HPA mRNA and HIF-1α protein in the esophageal squamous cell carcinoma was significantly correlated with fibromembranous infiltration and lymph node metastasis(P0.05). There was a close correlation between the expression of HPA mRNA and HIF-1α protein in the esophageal squamous carcinoma tissues(Kappa=0.615,P0.05).Conclusion: The high expression of HPA mRNA and HIF-1α protein is correlated with carcinogenesis progression,invasion and metastasis of esophageal squamous cell carcinoma. There is a synergic action between them in the carcinogenesis and development of esophageal carcinoma. The determination of HPA and HIF-1α will be useful for predicting tumor biological behavior.