The Expression of PCNA in Cervical Squamous Cell Carcinoma and Its Clinical Significance

Objective To investigate the significance of the overexpression of PCNA gene in cervical intraepithelial neoplasm (CIN) and squamous cell carcinoma. Mathods The paraffin slides were cut and stained with HE, and immunochemically stained with ABC method. Then, the PCNA/cyclin labeling index (PCNA LI) was counted under optical microscope. Results Form the normal cervical squamous epithelia to CINⅡand to squamous cell carcinoma, the scale of cells which expressed the PCNA positive and the intensity of PCNA expression tended to increase obviously. There was significant difference in their PCNA LIs ( P 0 05). Of the 45 cases of squamous cell carcinomas, the PCNA LIs increased as the grades of the carcinomas increased There was significant diffetence in their PCNA LIs ( P 0 05) There was also significant difference ( P 0 05) in the PCNA LIs of the cases with lymph hodes metastatic carcinoma and of those without metastatic carcinomas. Conclusions The PCNA LIs had great singificance of monitoring the occurrence and development of cervical carcinomas, making the early diagnosis and predicting the prognosis of the patients.