Apoptotic induction by mdm2 siRNA on human colorectal carcinoma cell lines LoVo

Objective To investigate the effect of mdm2 Small interfering RNA(siRNA) on apoptosis in human colorectal carcinoma cell lines LoVo cells.Methods LoVo cells were transfected with mdm2 siRNA.RT-PCR and Western blot analysis of mdm2 mRNA and protein expression in LoVo cells.Growth curve,Flowcytometry,TUNEL assay and MTT colorimetry were used for determination of apoptosis in LoVo cells.Results Transfection of LoVo cells with mdm2 siRNA significantly inhibited MDM2 expression at both mRNA and protein level in LoVo cells;treatment of LoVo cells with mdm2 siRNA resulted in growth inhibition;this significantly increased apoptotic cell rate.mdm2 siRNA had an effect on induction of either early or late stage apoptosis.We also found that when combined with DDP,mdm2 siRNA significantly inhibited tumor growth with reductions in tumor cells compared with that seen with mdm2 siRNA.Conclusion Mdm2 siRNA effectively inhibits mdm2 gene expression in LoVo cells and leads to induction of apoptosis in LoVo cells.And it also enhances the sensitivity to DDP.The use of mdm2 siRNA technique may provide a novel therapeutic approach to treat tumors.