Cloning and Sequence Analysis of S-RNase Gene from Citrus grandis var.Shatinyu Hort

In this paper,the transcriptome of the self-pollinated style and cross-pollinated style of Citrus grandis var.Shatinyu Hort were sequenced by high-throughput sequencing technology.S-RNase gene sequences of Citrus grandis var.Shatinyu Hort was obtained through different analytical method.It is1 238bp(GenBank accession number:KP172529)in length with an open reading frame(ORF)of 834 bp,encoding 278 amino acids with deduced molecular weight of 31.402 kDa,and theoretical pI value of5.30.Bioinformatics analysis showed that S-RNase is a hydrophilic protein,and there are 17 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the S-RNase protein shared high homology with that of Citrus maxima(99%),Citrus reticulate(98%)and Citrus sinensis(96%).Phylogenetic analysis revealed that S-RNase gene showed closer kinship with that of Citrus maxima、Citrus reticulate and Citrus sinensis,indicating that they belong to the same evolutionary branch.