Cloning and Sequence Analysis of RNase-like Gene FromCitrus grandis var.Shatinyu Hort

The self-pollinated and cross-pollinated style of Citrus grandis var.Shatinyu Hort were used as test materials and transcriptome sequenced by high-throughput sequencing technology.The sequence of RNase-likestorage protein was obtained by differential analysis,what's more,the physical and chemical properties of this protein were studied.The result showed that,the length of this gene(GenBank accession number:KP172529)was 1 048 bp with an open reading frame(ORF)of 693 bp,encoding 290 amino acids with deduced molecular weight of 25.82 kDa,and the theoretical pI value was 5.30.And it belonged to the RNase-T2 superfamily with a conserved region binding the nucleotide.This protein was a hydrophobic protein,and there were 15 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the RNase-like storage protein shared high homology with Citrus sinensis(99%)and Citrus clementina(99%).The phylogenetic tree revealed that RNase-like gene storage protein showed closer kinship with Citrus sinensis and Citrus clementina,indicating that they belonged to the same evolutionary branch.