Determination of Atorvastatin in Human Plasma by HPLC and Pharmacokinetics Study

OBJECTIVE:To establish the method for the determination of atorvastatin in human plasma and study its pharmacokinetics.METHODS:20 male healthy volunteers were given Atorvastatin tablets 10 mg orally.The plasma concentration of atorvastatin was determined by HPLC,and pharmacokinetic parameters were measured.RESULTS:The linear range of atorvastatin were 0.1～12.5 μg·mL-1,the intra-day and inter-day RSD were all 9%,the recovery test result were 89.00%～103.00%.The main pharmacokinetic parameters of atorvastatin were as follow:t1/2(14.40±7.10)h,tmax(1.50±0.70)h,cmax(6.10±3.40)μg·L-1,AUC0～48 h(50.60±43.60)μg·h·L-1,AUC0～∞(56.70±42.50)μg·h·L-1,MRT0～48 h(3.68±0.75)h,MRT0～∞(3.82±0.71)h.CONCLUSION:The method is suitable for pharmacokinetic study of atorvastatin in healthy volunteer.