Effects of Aloe vera and Compound Extract of Aloe vera on Inflammatory Factors in RAW264.7 Cells

Objective:To observe effect of the Aloe vera and its compound extract on inflammatory factors.Method: Cells diluted by 2×105/mL were inoculated into 96-well plates,100 μL per well,concentrations of druds was 250,125,62.5 g·L-1,MTT method was assay to test the toxic effects of macrophages.When the concentrations was 50,25,12.5 g·L-1 macrophage nitric oxide(NO) levels was measured.Enzyme-linked immunosorbent assay(ELISA) were used to measure tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) levels in pure drug and lipopolysaccharide(LPS)-induced murine macrophage.Result:Aloe vera and its compound extract promoted releasing of NO,with a concentration dependent manner(P0.01);Aloe vera could promote TNF-α secretion(P0.01) and inhibition of IL-10 secretion(P0.05),the compound inhibited LPS induced releasing of TNF-α and IL-10(P0.01).Conclusion: Aloe in 250-7.8 g·L-1 concentration has non-cytotoxic macrophages,compound extract has a certain toxic action,and can exert some influence on release of inflammatory factors.