Aloe vera Protective Effect on Lipopolysaccharide-Induced RAW 264.7 Inflamed Cells

During inflammation, the immune system releases proinflammatory mediators. Therefore, suppression of inflammatory mediators has been one of the most valuable strategies for treating inflammatory diseases. Research has shown that medicinal plants have anti-inflammatory properties. This study aimed to investigate the cytotoxicity and protective effect of Aloe vera gel extract (AVGE) on inflamed LPS-stimulated macrophage cells (RAW 264.7 ATCC TIB 71). Based on the cytotoxicity test, Aloe vera gel extract (AVGE) concentrations of 12.5 μg/mL and 75.0 μg/mL were chosen in this research as more than 90% cell viability was seen. Results showed that the level of critical inflammatory mediators observed in this study was significantly reduced by Aloe vera gel extract (AVGE) 75.0 μg/mL concentration and percentage inhibition over positive control, that is, PGE2(627.42 ± 27.77 pg/mL and 29.79 ± 3.11 pg/mL), TN α (265.7 ± 36.57 pg/mL and 54.24 ± 6.30 pg/mL), and IL-1β (191.33 ± 0.66 pg/mL and 56.23 ± 0.15 pg/mL). Microscopically, the cytokine production was reduced significantly post-treated with Aloe vera gel extract 75.0 micro g/mL concentration. These findings confirmed the protective effect of Aloe vera gel extract (AVGE) towards inflamed cells.