2001•Unpublished venueRequires access
Protective role of glycyrrhizin on experimental obstructive nephropathy in rats
Kai Li
Open publisher page 0 citations
Abstract
Objective To investigate the protective role of glycyrrhizin on experimental obstructive nephropathy in rats. Methods The model of obstructive nephropathy was induced by unilateral ureteral ligation in rats and the animals were sacrificed at 12 h and 3,14,28,56 d respectively after operation. Specimens were taken from the cortex of kidney. In renal stroma, routine morphological examinations and counts of karyocyte were made, the expression of collagen type Ⅰand type Ⅲ were also detected by immunohistochemical staining,then, semi quantified by EIG image analysis system. Results A progressive fibrosis was observed in renal stroma of the model. 12 h after operation, counts of karyocyte increased markedly in renal stroma in groups of treatment and pretreatment with glycyrrhizin compared with that in sham operation group( P 0.05),and decreased distinctly compared with that in saline treated group( P 0.01), but no significant difference was found between treatment group and pretreatment group( P 0.05);3 d after operation, in group treated or pretreated with glycyrrhizin. The expression of collagen type Ⅰ increased markedly compared with that in sham operation group( P 0.01) in renal stroma, but decreased notably compared with that in saline treated group( P 0.01),while, there was not any difference between treatment group and pretreatment group( P 0.05). The expression of collagen type Ⅲ in renal stroma was almost the same as that of collagen type Ⅰ. Conclusion Glycyrrhizin has some therapeutical but no preventive effect to experimental obstructive nephropathy in rats.