Enzyme Purification of β-1,3-glucan from waster beer yeast

The technology for enzyme treatment purifying β-1,3-glucan extraction from waster beer yeast was studied in this paper.208U/g(substrate) papain was added in the beer yeast and incubated for 8 hours at pH6.0 and 60℃,protein removal rate would be 62.82%,the final purity and yield of β-1,3-glucan were 90.50% and 11.00%.It was proved that a high-purity β-1,3-glucan was obtained through the UV、TLC and properties analysis,0.348g/L protein hydrolyzates con-sisted of polypeptide and amino acid were also recycled.