Determination of α-2,5-Dimethoxyphenyl-β-aminoethanol Hydrochloride in Serum by HPLC with Fluorescence Detection

A HPLC method with fluorescence detection for the determination of α 2,5 dimethoxyphenyl β aminoethanol hydrochloride in serum was established. The serum sample was treated with solid phase extract. A Diamonsil C 18 column was used, with the mobile phase of methanol 0.1% trifluoroacetic acid solution (28∶72). The excitation and emission wavelength were 288 and 328 nm, respectively. Methoxamini was used as internal standard. The calibration curve was linear in the range of 0.5~32 ng/ml ( r =0.9999). The limit of detection was 5 pg. The extraction recovery was more than 86%, and the RSD of intra and inter batch were 1.2%~5.4%.