Effects of Different Traditional Chinese Medicines on Hippocampal Mitochondrial Membrane Potential After Intracerebral Hemorrhage in Rat Models

Objective To study the effects of various TCMs treatment on the changes of mitochondrial membrane potential (MMP) after intracerebral hemorrhagic (ICH) injury in rat models, and to explore the therapeutic mechanism of PGXF decoction. Methods Ⅶ type collagenase was stereotaxically injected into the globas pallidus of rats to induce intracerebral hemorrhage and spectrofluorometer was used to detect and monitor the fluorescence value changes occurred in the mitochondrial suspension, and the neurobehavioral scores were used to evaluate the neurological defect. Results 1.Neurological defects were observed after ICH in rat models and the scores of neurological defects in model group received various TCMs intervention reached to peak values 4 h after onset of hemorrhage, and then decreased gradually, but were still higher than those in sham-operation group and normal group on the 7th day (P0.01). The equation scores of the treatment groups were lower than the model group on the 1st, 3rd and 7th days. The scores of Pinggan Xifeng decoction group were lower than the model group and other TCMs groups on the 3rd and 7th days (P0.05). 2. The MMP of ICH model groups reduced significantly 4 h after onset of hemorrhage, peaked at the 1st day and then still reduced as compared with the sham-operation group and normal group during the 7th day(P0.01). The MMP in the PGXFD group was significantly higher than the model group and other treatment groups on the 3rd and 7th days (P0.05). Conclusions MMP decreased in rats experienced with experimental ICH. Various TCM therapies can depress the decrement of MMP and improve the neurological defects, particularly Pinggan Xifeng decoction shows the best benefit.