The effects of Exogenous VEGF on cerebral angiogenesis and nerve functional recovery in rat with intracerebral hemorrhage

Objective To study the effects of exogenous VEGF on cerebral angiogenesis,the expression of GAP-43 and nerve functional recovery in rat with intracerebral hemorrhage (ICH),to reveal the mechanism of neuroprotection by exogcnous VEGF.Methods The model of ICH was established in rats by two - step intracerebral injection of autogenous blood.36 male Spranue - Dawley rats were randomly divided into sham- operated group,ICH model group and treatment group.The treatment group was inject with VEGF intraperitoneally during postoperative Day8 to Day15.The expression of CD34,GAP- 43 were detected by immunohistochemistry on Day15 and Day30.Microvascular production was measured by expression changes of CD34.Neurotrosis and neural Repair was measured by expression changes of GAP -34 and Longa evaluation.Results the expression of CD34 + microvascular density and vascular field area of treatment group in Day15 and Day30 are significantly higher than those of model group and sham -operated group ( P ＜ 0.01 ).The rate of positive cell of GAP -43 and Positive particles area of the treatment group in 15 d and 30 d are significantly increased,compared to the model group and the sham - operated group.Longa evaluation showed significant difference among model group and treatment group in Day15.Longa evaluation of treatment group was better than thoes of model group.Conclusions Exogenous VEGF could enhance angiogenesis and up - regulate the expressions of GAP - 43 after intracerebral hemorrhage.It could reduce Neurotrosis caused by intracerebral hemorrhage and improve neurological function. Key words: Exogenous VEGF; Intracerebral hemorrhage; CD34; GAP43