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2008•Xibei nongye xuebaoRequires access

Processing Tomato Plant Regeneration in Genetic Transformation

Jianxin Niu

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Abstract

When tomato seedlings grew 7~8 days after germination cotyledons were cut into 0.2~0.4,0.3~0.5 cm discs as explants.MS+B5 was used as basic medium,and IAA0.2 mg/L combined with different concentrations of 6-BA/ZT/TDZ on bud induction;6-BA2.0 mg/L combined with different concentrations of IAA /IBA/NAA on bud induction.The results showed that the best shoot induction medium for cotyledon explants was MS+ZT1.0 mg/L +IAA0.2 mg/L and MS+TDZ2.0 mg/L+IAA0.2 mg/L in different combinations of IAA0.2 mg/L and 6-BA/ZT/TDZ.Among different combinations of 6-BA2.0 mg/L and IAA/IBA/NAA,IAA was bester than that of IAA and IBA on bud induction.The best medium on bud induction was MS + 6-BA 2.0 mg/ L + IAA 1.0 mg/ L.The root could be induced after the regenerated buds were planted in M S+ IBA 0.5 mg/L one week later.

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What this paper is about

When tomato seedlings grew 7~8 days after germination cotyledons were cut into 0.2~0.4,0.3~0.5 cm discs as explants.MS+B5 was used as basic medium,and IAA0.2 mg/L combined with different concentrations of 6-BA/ZT/TDZ on bud induction;6-BA2.0 mg/L combined with different concentrations of IAA /IBA/NAA on bud induction.The results showed that the best shoot induction medium for cotyledon explants was MS+ZT1.0 mg/L +IAA0.2 mg/L and MS+TDZ2.0 mg/L+IAA0.2 mg/L in different combinations of IAA0.2 mg/L and 6-BA/ZT/TDZ.Among different combinations of 6-BA2.0 mg/L and IAA/IBA/NAA,IAA was bester than that of IAA and IBA on bud induction.The best medium on bud induction was MS + 6-BA 2.0 mg/ L + IAA 1.0 mg/ L.The root could be induced after the regenerated buds were planted in M S+ IBA 0.5 mg/L one week later.

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Available abstract

When tomato seedlings grew 7~8 days after germination cotyledons were cut into 0.2~0.4,0.3~0.5 cm discs as explants.MS+B5 was used as basic medium,and IAA0.2 mg/L combined with different concentrations of 6-BA/ZT/TDZ on bud induction;6-BA2.0 mg/L combined with different concentrations of IAA /IBA/NAA on bud induction.The results showed that the best shoot induction medium for cotyledon explants was MS+ZT1.0 mg/L +IAA0.2 mg/L and MS+TDZ2.0 mg/L+IAA0.2 mg/L in different combinations of IAA0.2 mg/L and 6-BA/ZT/TDZ.Among different combinations of 6-BA2.0 mg/L and IAA/IBA/NAA,IAA was bester than that of IAA and IBA on bud induction.The best medium on bud induction was MS + 6-BA 2.0 mg/ L + IAA 1.0 mg/ L.The root could be induced after the regenerated buds were planted in M S+ IBA 0.5 mg/L one week later.

Key concepts: Cotyledon, Explant culture, Germination, Shoot, Horticulture, Botany, Biology, Murashige and Skoog medium

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