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Cloning and Analysis of cDNA Sequences of 4.5S RNAs of Rat Cells

Lai Xu

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Abstract

The cDNA of 4.5S RNAs were amplified by reverse transcription\|polymerase chain reaction (RT\|PCR) method using total RNA extracted from the liver tissue of rat as a template. The cDNA fragment was inserted into pGEM3Zf (+) plasmid and cloned to obtain the recombinant pGEM3Zf (+)\|4.5S.Using enzyme digestion and polyacrylamide gel electrophoresis methods, the recombinant plasmids that contained 4.5S cDNA sequences were detected. Then the cDNA sequences of rat 4.5S RNAs were sequenced, and the results were compared with 4.5S RNAs sequence of reported mouse and hamster to find its differences and analyzed the structural characters.

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What this paper is about

The cDNA of 4.5S RNAs were amplified by reverse transcription\|polymerase chain reaction (RT\|PCR) method using total RNA extracted from the liver tissue of rat as a template. The cDNA fragment was inserted into pGEM3Zf (+) plasmid and cloned to obtain the recombinant pGEM3Zf (+)\|4.5S.Using enzyme digestion and polyacrylamide gel electrophoresis methods, the recombinant plasmids that contained 4.5S cDNA sequences were detected. Then the cDNA sequences of rat 4.5S RNAs were sequenced, and the results were compared with 4.5S RNAs sequence of reported mouse and hamster to find its differences and analyzed the structural characters.

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Available abstract

The cDNA of 4.5S RNAs were amplified by reverse transcription\|polymerase chain reaction (RT\|PCR) method using total RNA extracted from the liver tissue of rat as a template. The cDNA fragment was inserted into pGEM3Zf (+) plasmid and cloned to obtain the recombinant pGEM3Zf (+)\|4.5S.Using enzyme digestion and polyacrylamide gel electrophoresis methods, the recombinant plasmids that contained 4.5S cDNA sequences were detected. Then the cDNA sequences of rat 4.5S RNAs were sequenced, and the results were compared with 4.5S RNAs sequence of reported mouse and hamster to find its differences and analyzed the structural characters.

Key concepts: Complementary DNA, Molecular biology, Recombinant DNA, Biology, Cloning (programming), Plasmid, Rapid amplification of cDNA ends, RNA

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