Effects on endothelial cell proliferation induced by different concentration of iodine in vitro

Objective:To research the effects on proliferation of human umbilical vein endothelial cells(HUVECs) induced by different doses of iodine.Methods: HUVECs were subjected to iodine in various concentrations(0.5,1.0,2.0,3.0,4.0,5.0,6.0,and 8.0?μmol/L)for 24 hours,and the morphology of the cultured endothelium was observed.MTT colorimetry was used to determine the proliferation activities of the cultured endothelium.Results: Iodine significantly increased the number and MTT activities of vascular endothelial cells from 0.5?μmol/L to 4.0?μmol/L compared with the normal group at 24 hours.Cell morphology was normal under a microscope.The number and MTT activities of vascular endothelial cells were significantly increased by iodine at over 5.0?μmol/L at 8 hours,but were decreased at 12 hours and later.Poison granules in the endothelium were produced by iodine at over 5.0?μmol/L at 4 hours and increased in a dose-and time-manner.Conclusion: Iodine effects on the proliferation activity of endothelial cells are dose-and time-dependent.