Effects of Qingyitang on lung injury caused by severe acute pancreatitis in rats

[Objective] To investigate the effect of Qing yitang on acute lung injury(ALI) induced by severe acute pancreatitis(SAP).[Methods]SD rats were randomly divided into 3 groups: sham operation group(SO,n=10),SAP model group(SAP,n=10),and Qing yitang group(QYT,n=10).Sham operation was only made in SO.Severe acute pancreatitis was induced by retrograde injection of 1.5% deoxidat sodium cholat acid to pancreatic duct in SAP and QYT.Qing yitang(10ml/kg) was intragastricly administered 30min and 12h after SAP was induced in QYT group.Serum amylase(AMY) levels,pH,PaO2,PaCO2 and lung wet/dry ratio(W/D) were determined.SP-A mRNA expression in lung was detected by RT-PCR.The pathologic changes of pancreas,lung were observed 24 h after establishment of the model.[Methods]Serum levels of AMY(7144.19±727.91) U/L,W/D(8.57±2.45)in SAP were markably higher than that in SO(1193.41±192.54) U/L,(3.70± 0.90) and QYT(4283.51± 527.52) U/L,(4.05± 0.52)(P0.01).The levels of PaO2 in SAP(79.24±5.84)mmHg were lower than that in SO (96.78±3.81)mmHg and QYT(88.16± 5.07) mmHg(P0.05,0.01).The expression of SP-A mRNA in lung in SAP was significantly decreased than in that in SO and QYT(P0.01).The pathologic changes of pancreas,lung in QYT and SO were milder than in SAP.[Conclusion ]The expression of SP-A decreased markably in ALI induced by SAP.Administration of Qing yitang can reduce the injury of lung by restituting the expression of SP-A.