Effect of thoracic duct ligation/conduction in rats with severe acute pancreatitis accompanied with acute lung injury

Objective To investigate the effects of thoracic duct ligation/conduction in rats with severe acute pancrtitis (SAP) accompanied with acute lung injury (ALI).Methods Models of SAP accompanied with ALI were made through retrograding injection into biliopancreatic duct by 5％ sodium taurocholate,then thoracic duct was conducted or ligated after separation. Effects of different operations on lung ultrastructure,lung pathogenesis score,blood gas and the expression of Survivin were tested.Results As compared with SO group at the same time point,lung pathogenesis score was increased significantly in SAP,LC and DC groups (P ＜ 0.01 ). Alveolar type Ⅱ cells changed more obviously in SAP and LC groups than in DC groups under the transmission electron microscopy (TEM).As compared with SO group at the same time point,PaO2 in subgroups of SAP 2 h and SAP 6 h was also increased significantly ( all P ＜0.01 ),PaO2 in subgroup SAP 2 h was obviously higher than in subgroup of SAP 6 h ( all P ＜0.01 ),and PaO2 in subgroup of SAP 12 h was obviously higher than in subgroup of SO 12 h (P ＜0.01 ).As compared with SO group at the same time point,PaCO2 in subgroups of SAP 2 h and SAP 6 h was decreased significantly ( P ＜ 0.01 ),and PaCO2 in subgroup of SAP 12 h was obviously higher than in subgroup of SO 12 h (P ＜ 0.01 ).The effects of conduction were more satisfactory than those of ligation.The expression of Survivin was stronger in SAP group ( 1.278 ± 0.172 ) than in SO group ( 0.568 ± 0.077 ) ( P ＜ 0.05 ) at 2 h.The expression of Survivin in LC group (0.624 ±0.084) and DC group (0.282 ±0.038) was weaker than that in SAP group ( 1.074 ± 0.145 ) at 12 h ( both P ＜ 0.05 ),and th expression of Survivin in DC group was decreased more obviously.Conclusion Thoracic duct ligation or conduction could decrease the pathological changes in rats with SAP,and the effect of conduction was more satisfactory than that of ligation. Key words: Acute pancreatitis; Acute lung injury; Thoracic duct ligation/conduction; Survivin