2012•Zhongguo yaofangRequires access

Content Determination of Osthole in Sanpi Zhixuan Tincture by RP-HPLC

Chengxiao Fu, Long Yu, Qi Liu

Abstract

OBJECTIVE: To establish a method for the content determination of osthole in Sanpi zhixuan tincture. METHODS: RP-HPLC method was adopted. The determination was performed on Ultimate XB-C18(250 mm×4.6 mm, 5 μm) column with mobile phase consisted of methanol-water(76∶24,V/V) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 322 nm and column temperature was 25 ℃. RESULTS: The linear range of osthole was 0.038 4～0.768 0 μg(r=0.999 6)with an average recovery of 100.45%(RSD=1.88%,n=6). CONCLUSION: The method is simple, rapid, accurate and suitable for the content determination of osthole in Sanpi zhixuan tincture.

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Key concepts: Tincture (heraldry), Chromatography, Content determination, High-performance liquid chromatography, Linear range, Chemistry, Linear relationship, Detection limit