Changes of Lymphocyte Subpopulation and Its Significance in the Children with Kawasaki Disease During Acute Phase

Objective:To investigate the change and significance of lymphocyte subpopulation in the children with kawasaki disease(KD) during acute phase. Method:Two hundred and nineteen patients diagnosed as KD in the hospital were analyzed retrospectively,106 healthy children were chosen as control. Flow cytometric analysis was employed to detect the percentage of lymphocyte subpopulation(including CD3+cells、CD3+ CD4+cells、CD3+CD8+cells 、CD16+CD56+cells、CD19+cells)and CD4+/CD8+ratio in the KD patients before treatment and in the healthy children. Result:Compared with healthy control,the percentage of CD19+cells、and CD4+/CD8+ratio in patients with KD increased significantly(P0.01),and the percentage of CD3+CD8+cells、CD16+CD56+cells decreased significantly(P0.01),but there were not any significant change about the percentage of CD3+CD4+cells(P=0.24). Based on the standard value of lymphocyte subpopulation in the hospital,there were 199 KD patients who had increased CD19+cells percentage,but only 11 children out of the healthy group had higher CD19+cells percentage(P0.01). Conclusion:There are evident immunity disturbances during the acute phase of KD,these changes including decreased percentage of CD3+CD8+cells,CD16+CD56+cells and elevated CD19+cells percentage might take some role in the KD development,and detecting the percentage of lymphocyte subpopulation maybe helpful in the diagnosis of KD from one immunological view.