Full-length cDNA Cloning of Glycosyltransferase Family from Rhodiola sachalinensis

In order to obtain the critical enzyme for the synthesis of Rhodiola sachalinensis glycoside,two putative UDPglycosyltransferase(UGT) cDNAs(GenBank accession number,EF508689 和 EU567325) were isolated from R.sachalinensi.The primers were designed for 3 '-rapid amplification of cDNA ends(RACE) based on the consensus hybrid oligonucleotide primers(CODEHOP) strategy by the Block Maker program.A full-length cDNA sequence(Genbank accession numbers:EF508689 and EU567325) was obtained through the amplification using a SMARTerTM RACE cDNA Amplification Kit(TaKaRa,Dalian,China),whereas the sequence obtained using a 5'-RACE system(version 2.0,InvitrogenTM Life Technologies) contained no full-open reading frame.The cloning efficient of two 5'-RACE systems was investigated.Results exhibited that TaKaRa SMARTerTM RACE cDNA Amplification Kit was more efficient in complete cDNA sequence cloning,compared with the InvitrogenTM 5'-RACE system.