Screening for antimicrobial peptide genes in scallop by improved PCR method of rapid amplification of 3' cDNA ends

Objective To screen antimicrobial peptide genes from scallop with improved PCR method of 3' rapid amplification of cDNA ends(RACE).Methods Gene special primers and anchor primers modified by promoters M13 and T7 were designed.Total RNA was extracted from scallop and amplified by improved PCR method of 3' RACE and 5' RACE to obtain the full-length cDNA of antimicrobial peptide genes.The homology of the sub-full-length cDNA sequence was blasted in GenBank.Results Three 3' end cDNA sequences and one sub-full-length cDNA(544 bp) were obtained.There was no similar gene in homology in GenBank.Conclusion One sub-full-length cDNA and three cDNAs 3' end sequences from scallop hemolymph may be new genes.It is available to use improved PCR method of RACE to screen gene fragments containing antimicrobial peptide conserved sequence.