The quantitative analysis of p16protein expression in glioma development and progression

Objective To study the role of p16protein in glioma development,progression and recur-rence.Methods The p16protein expression was determined in normal cerebral tissue,peritumoral tissue and different grade gliomas by LsABC(labelled streptavidin biotin method)immunohistochemical method,and its distribution areas were measured by image analyzing system;the expression alternations in pre-and post-re-currence of tumors and the correlation with recurrent time were statistically analyzed.Results1)The ex-pression of p16protein decreased in the order of normal cerebral tissue,peritumoral tissue and glioma,the distribution areas were(613.43±217.21)μm 2 ,(560.26±202.89)μm 2 and(307.24±116.91)μm 2 respectively(P0.05or P0.01);2)The p16protein expression decreased as the pathological grade increased,the distribution areas of astrocytoma (gradeⅠ-Ⅱ),anaplastic astrocytoma (gradeⅢ),and polymorphic primary glioblastoma (gradeⅣ)were(491.91±215.32)μm 2 ,(396.83±201.87)μm 2 and(203.67±143.19)μm 2 respectively(P0.05or P0.01);3)The distribution areas of p16protein expression decreased in recurrent tumors,especially the p16protein expression area difference was significant higher in those with increased grade(103.152±20.829)μm 2than that in unchanged grade(59.381±16.215)μm 2 (P0.01);4)The recurrent interval time was negatively correlated with p16protein expression level(r=?0.453,P0.01).Conclusion The p16protein expression is correlated with progression and recurrence of gliomas,thus further study on mechanism of its expression alter-nation will be helpful to the prevention and treatment of glioma recurrence.