Physico-chemical Characteristics and Antioxidant Activity of Polysaccharide from Ganoderma atrum Fruitbody

In order to investigate physico-chemical characteristics and antioxidant activity of polysaccharide from Ganoderma atrum fruitbody,a pure polysaccharide was isolated and purified sequentially using Sevag method,ethanol grade precipitation and Superdex-200 gel filtration chromatography. The purity of this polysaccharide was identified by HPLC. Solubility,thermal stability and spectral properties of this polysaccharide were also evaluated. Meanwhile,scavenging capability of hydroxyl free radicals,total antioxidant capability and suppression ability in β-carotene linoleic acid oxidative system were analyzed. It was concluded that polysaccharide from Ganoderma atrum has distinct antioxidant activity,which provides a theoretical basis for the exploitation of a new natural antioxidant.