Analysis on Composition and Genetic Variations of HMW-GS of Some Winter Wheat Varieties in Longdong

High molecular weight glutenin subunit composition and frequencies of some varieties in Longdong were analyzed by SDS-PAGE techniques.The results showed that there were 16 HMW-GS subunits combinations in tested materials.The subunits of N,7+8,2+12 were major type account for 36.50% of whole varieties.But there were only 3 subunits combinations of 5+10 subunit,account for 9.62% of whole varieties.HMW-GS subunit composition characteristics and frequency showed that there was lower genetic diversity in Glu-1 locus,Glu-D1 locus encoded 6 allelic variations,and the frequency of N subunit was the highest(71.15%).Glu-B1 locus encoded four allelic variations,the frequency of 7+8 was the highest(59.62%).Glu-A1 locus encoded 2 allelic variations,and the frequency of 2+12 was the highest(61.4%).Subunits combinations of the landrace variety presented the characteristics of simple composition and lacked high quality subunits.But the modern variety and introduced variety had comparatively abundant subunits combinations which contained certain high quality suunits.