Effects of Trans-resveratrol on the Expression of NMDAR1 and NMDAR2A after Injury in Rat's Hippocampus by Aβ_(25-35)

Objective To observe the effects of trans-resveratrol(TR) on the expression of NMDA receptor NR1 and NR2 A subunits in hippocampal Aβ25-35 injured rat. Methods After being trained using Morris water maze(MWM), the qualified rats were selected and randomly divided into 6 groups: sham-operated group, model group, TR high dose group, TR middle dose group and TR low dose group, Donepezil group. The model group was established by injecting Aβ25-35 to hippocampus of the rats. After the model was established, it was treated with TR of high dose, moderate dose and low dose to make TR high, middle and low dose group, and Donepezil to make Donepezil group. Each group has 6 rats. Real time PCR was used to detect the mRNA expression of inducible nitric oxide synthase NMDAR1, NMDA R2 A in hippocampus of the 6 groups of rats. Results The escape latency of model group was significantly longer than that of TR high, middle, low dose group, Donepezil group and sham-operated group in MWM. The mRNA expression of NR1 in each group was 0.14 ± 0.04, 1.51 ± 0.54, 0.22 ± 0.06, 0.37± 0.08, 0.61 ± 0.15(LOW),0.97 ± 0.16(Donepezil), respectively, and the mRNA expression of NR2 A was 0.12±0.07, 1.72±0.34, 0.16±0.06, 0.21±0.08, 0.52±0.15, 0.67 ±0.16, respectively. Conclusion TR may downregulate the mRNA expression of NMDA R1 and NMDAR2 A in hippocampus, thereby reducing the cell apoptosis in hippocampus.