Protective effects of trans-resveratrol on hippocampal neurons in the rats with Aβ_(25-35) induced Alzheimer's disease

OBJECTIVE To investigate the protective effects of trans-resveratrol( TR) on hippocampal neurons of rats with Aβ25-35induced Alzheimer's disease( AD),and explore its possible mechanisms. METHODS Rats are randomly divided into sham,positive control( donepezil),model( Aβ25-35),low and high dose TR groups,respectively. After injection of Aβ25-35into hippocampus,rats were administrated with 1. 0 mg·kg-1donepezil and 10 and 40 mg·kg-1TR once daily by gavage for consecutive 15 days respectively,while sham and model groups were administrated with volume-matched normal saline by gavage. The learning and memory ability of rats were detected by Morris water maze test. Neuronal injury in hippocampus was observed by hematoty-eosin staining,and the mRNA levels of APP were detected by real time PCR and the protein expressions of caspase 8 were examined by immunohistochemistry and Western Blot in the rat hippocampus. RESULTS Compared with the model rats,TRes-treated groups significantly shortened the mean escape latency in the navigation test and prolonged the adjusted escape latency in spatial probe test,attenuated neuronal injury in hippocampus,and significantly decreased the mRNA expressions of APP and the proteins of caspase 8 in rat hippocampus.CONCLUSION TR has protective effect of on hippocampal neurons of rats with Aβ25-35induced AD,its mechanisms may be at least partly due to decreasing the mRNA expressions of APP and protein expression of caspase8 in rat hippocampus.