Study on the Callus Induction of Blackcurrant

Taking the plantlet of two varieties of blackcurrant‘Hanfeng'and‘Fortodi'as materials,with the leaves,the petioles and the stems as explants,the effect of explants on callus induction rate was studied,and the influence of different hormones and concentrations on callus induction,callus subculture and plant regeneration were investigated.The results showed that the petioles was the best explants for callus induction.The different concentration of 2,4-D that was used in callus growing had obvious effect on the callus differentiation,and the optimal concentration of 2,4-D was 1.0mg/L.Callus could be induced by culturing leaves,petioles and stems of blackcurrant in vitro.The young stems could preliminary differentiate the adventitious buds in a suitable medium through callus.It laid a foundation for the blackcurrant callus regeneration and genetic transformation.