Isolation and Identification of Alkaloids from Macleaya microcarpa(Maxim.) Fedde

The method of isolation of total alkaloids from the acidic extraction solution of Macleaya microcarpa was established by using D101 macroporous resin as adsorbens to provide free alkaloid fraction A with 50% ethanol in water as eluent and free alkaloid fraction B with acetone as eluent.From the fraction A,five isoquinoline alkaloids,dihydrosanguinarine(1),dihydrochelerythrine(2),6-ethoxysanguinarine(3),6-acetonyl-dihydrosanguinarine(4) and Bocconarborine A(5) were isolated,and from the fraction B,protopine(6),α-allocryptopine(7) and β-allocryptopine(8) and berberrabine(9) was obtained,of which 3,4,5 and 9 were found in this plant for the first time.Their structures were determinated on the basis of spectroscopic evidence.