Protection Effects of Flavonoids from Morus alba Leaves on Carbon Tetrachloride Induced Liver Injury in Mice

Objective: To study the protection effects of Flavonoids extraction from Morus alba leaves(FE) on carbon tetrachloride(CT) induced liver injury mice and investigate the possible mechanisms.Method: Kunming mice were randomized into six groups(n = 10,each),The blank group,the model group,bifendate group(100 mg.kg-1),high dose of FE group(200 mg.kg-1),middle dose of FE group(400 mg.kg-1) and low dose of FE group(800 mg.kg-1).Mice were orally administered corresponding drugs one time daily for 14 days,the same volume of saline was infused in blank group and model group.The treated groups and model group were ip given 0.15% CCl4 in peanut oil(0.01 mL.g-1) once to produce acute liver injury on the 15th day.The morphological changes of the liver tissue were oberserved by HE stain.the serum activity of alanine transaminase(ALT),aspartate transaminase(AST) and the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and content of malondialdehyde(MDA) in liver were detected by spectrophotometry.Result: Compraed with the model group,the activity of ALT,AST and content of MDA in serum were obviously decreased(P 0.01),the activity of SOD,GSH-Px were increased markedly(P 0.01).Conclusion: FE has protective effects against acute liver injury induced by CCl4 in mice.