Effect of Leptin on Interleukin-6 Expression in Vascular Endothelial Cells

Objective To investigate the effect of leptin on the expression of Interleukin 6 in vascular endothelial cells.Methods Vascular endothelial cells were culture by primary cell culture,100 umol/L leptin was used to stimulate endothelial cells for 0,1,3,6,12 h. Then ELISA method was used to detect the expression of IL-6 protein, and the expression of IL-6 mRNA in vascular endothelial cells was detected with real-time fluorescence quantitative polymerase chain reaction(real-time PCR). Results Compared with the blank control group,the expression levels of IL-6 were higher in the leptin stimulation group, and the expression levels of IL-6protein and mRNA were increased when the action time extended.Conclusion Leptin can increase the expression and secretion of IL-6 in vascular endothelial cells in time-dependent manner.