Cloning and Expression Analysis of Fructokinase Gene(FRK) from Tomato Fruit

PCR primers were designed which based on the consensus domain of some fructokinase genes from tomato registered in GenBank.The cDNA fragments were obtained by reverse transcriptional polymersae chain reaction(RT-PCR)using the whole RNA of tomato,Liaoyuan Duoli(Solanum lycopersicum),as the templates.The fragments were cloned into pBS-T vector and then sequenced.The sequencing results showed highly homology(above 99%) in nucleotide sequence with the published sequences of tomato.A phylogenetic tree of FRK genes from Arabidopsis thaliana,Solanum tuberosum,Nicotiana tabacum and Solanum lycopersicum was constructed.The expression patterns of the cDNAs were examined in tomato fruit during different stages by semi-quantitative RT-PCR.FRK1-FRK3 was expressed in the whole stage of tomato fruit development,and strongly expressed from 25 to 35 days after anthesis.