Simultaneously Determine the Contents of Gallic Acid and Protocatechuic Acid in Mongolian Medicine Compound of Sendeng-4by HPLC

A HPLC method is established for simultaneously determining the contents of gallic acid and protocatechuic acid in Mongolian compound medicine of Sendeng-4.The chromatography were carried out in an mobile phase of acetonitrile(A)and 0.3% phosphoric acid aqueous solution at a flow rate of 0.7mL/min under the column temperature of 27℃ and the detection wavelength of 270 nm using a Elite-C18column(250mm×4.6mm,5μm).This method allowed the effective separation of gallic acid and protocatechuic acid with a good linearity in the detection range.The accuracy and repeatability were satisfactory and the average recoveries attained 100.5%(RSD=1.46%)and 97.0%(RSD=1.61%).The verification results revealed that this method can simultaneously,simply,rapidly,and accurately determine the contents of gallic acid and protocatechuic acid in Sendeng-4.