2014•Linchan huaxue yu gongyeRequires access
Production of L-Lactic Acid by Self-immobilized Rhizopus oryzae
Chen Xiao-pe
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Abstract
The main factors affecting self-immobilization of Rhizopus oryzae NLX-M-1 for L-lactic acid production were studied in present study. The optimum fermentation conditions utilizing wood fiber-based glucose for R. oryzae self-immobilization were obtained. The optimal results were achieved as 100 g /L initial glucose,2 g /L( NH4)2SO4,2 %inoculum size( value fraction),30 g /L CaCO3,0.1 g/L KH2PO4,0.25 g/L MgSO4·7H2O and 0. 1 g /L ZnSO4·7H2O. Under this optimal condition,R. oryzae formed pellets with an average diameter of about 1 mm during the fermentation,the production of L-lactic acid was 76. 6 g /L and the conversion rate was 81. 6 %. R. oryzae in acid explosive residue hydrolyzate containing 60 g /L glucose became 1. 2 mm pellets,and produced 36. 4 g /L L-lactic acid with a conversion rate of 63. 5 %.