Expression of c-fos Protooncogene Protein (FOS) in the Rat Brain After Cerebral Ischemic Pretreatment

Objective To investigate the FOS expression changes in rat brain after ischemic pretreatment and the relationship between FOS and ischemic tolerance.Method The cerebral ischemia model of the wister rats was established by occluding four blood vessels.The FOS experssion in the hippocampus neurons was determined by using SABC immunocytochemical technique with against c-fos protooncogene antibody of rabbit.Results The FOS expression in the 30',12h,24h and 72h four experimental groups following cerebral ischemic pretreatment were obviously higher than that of false operafion control group (all P0 01),especially 72h group,its expression levels was significantly higher than that of other three expeimental groups(P0 01).Conclusion After transient 3 minutes non-injury cerebral ischemia,FOS expression in rat brain was significantly increased following reperfusion 72 hours,which indicate that the FOS expression in brain might be one of the important factors inducing cerebral ischemic tolerance mechanism.