Expression and significance of MCM2 and Rb protein in laryngeal squamous cell carcinomas

Objective To study the expression and clinical significance of minichromosome maintenance protein 2(MCM2) and retinoblastoma susceptibility gene(Rb)protein in laryngeal squamous cell carcinomas(LSCC).Methods The MCM2 and Rb protein were detected in 60 cases of LSCC,10 cases of precarcinoma,10 cases of vocal cord polyps and 10 cases of normal laryngeal tissues by immunohistochemical staining.Results The positive expression rate of MCM2 in normal laryngeal tissues,vocal cord polyps,precarcinoma,laryngeal carcinoma were 10.00%,10.00%,30.00% and 85.00% respectively.The positive expression rate of MCM2 in LSCC was significantly higher than that in precarcinoma and normal laryngeal tissues(P0.05),and was positively correlated with pathological grades,clinical stages and lymph node metastases in LSCC(P0.05).The positive expression rate of Rb protein in normal laryngeal tissues,vocal cord polyps,precarcinoma,laryngeal carcinoma were 80.00%,80.00%,60.00% and 36.67% respectively.The positive expression rate of Rb protein in LSCC was significantly lower than that in precarcinoma and normal laryngeal tissues(P0.05).The expression of MCM2 in LSCC was negatively corelated with Rb protein(r=-0.542,P0.05).Conclusions Over expression of MCM2 and loss of Rb protein were related to the carcinogenesis and development of LSCC.The determination of MCM2 can be an index for estimating the degree of malignancy and prognosis of LSCC.