Effects of Celecoxib on NF-κB and iNOS in Rat Model of Acute Lung Injury

ObjectiveTo investigate the effects of celecoxib on the expressions of nuclear factor-κB(NF-κB) p65 and inducible nitric oxide synthase(iNOS) in rat model of acute lung injury induced by lipopolysaccharide(LPS).Methods Sixty rats were randomly divided into control group,LPS group,treatment group and celecoxib group(15 rats for each group).To copy the rat model of acute lung injury,LPS(5 mg/kg)was injected into the tail vein in LPS group.Celecoxib(20 mg/kg) was administered by gavage after 30-minute modeling in treatment group.Celecoxib(20 mg/kg)was also administered by ga vage in celecoxib group.The same volume of normal saline was treated in control group.Rats were sacrificed after 3 h in four groups.The expressions of cyclooxygenase-2(COX-2),NF-κB p65 and iNOS protein were detected by Western blot analy sis.The expressions of NF-κB p65 and iNOS mRNA were evaluated by reverse transcription polymerase chain reaction(RTPCR) assay.ResultsCompared with control group,expression levels of COX-2,NF-κB p65,iNOS protein,NF-κB p65 and iNOS mRNA were significantly higher in LPS group(P 0.01).The NF-κB p65,iNOS mRNA and protein expressions were significantly decreased in treatment group than those of LPS group(P 0.01).ConclusionThe selective COX-2 in hibitor celecoxib has a protective effect on acute lung injury in rats.