Isolation of a Naphthalene-degrading Strain and Analysis on Its Growth Characteristics

[Objective] The purpose was to study the degradation and transformation law of microbe on naphthalene.[Method] A naphthalene-degrading strain with higher activity was screened out from the active sludge from sewage treatment plant and its growth characteristics and naphthalene-degrading capability were studied.[Result] A naphthalene-degrading strain with higher activity was isolated from the soil sample A and it was named as TN-01.The preliminary identification indicated that it belong to Curtobacterium.The strain TN-01 had obvious lag phase,grew slowly in 0-15 h,entered into logarithmic growth phase after 16 h and entered into stable growth phase after 22 h.Its degradation rate on naphthalene reached 80.5% after 120 h.Under neutral and weakly basic conditions,the degrading effect of the strain TN-01 on naphthalene was better.When the pH value was 7.0,the degradation rate on naphthalene was highest.When the concentration.of naphthalene was 1 200 mg/L,the degradation rate on naphthalene was highest.When the inoculation amount was 1%,the strain TN-01 grew best.The degradation rates of the strain TN-01 on toluene and anthracene after 120 h were 76.8% and 79.4% respectively.and they were a little lower than that on naphthalene.[Conclusion] This research supplied reference data for controlling industrial naphthalene pollution.