2006•Zhongguo fuyou baojianRequires access
Cisplatin induced apoptosis in human cervical carcinoma Hela cells and its influence on cell cycle
Yu Wang
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Abstract
Objective:To investigate the effect of cisplatin on apoptosis and cell cycle of cervical carcinoma Hela cells in order to find out the mechanism of its anticancer action.Methods:By technique of cell culture in vitro,Hela cells were treated with cisplatin in different concentrations for 72 hours,then apoptotic rate and cell cycle were measured by flow cytometer.Results:Cisplatin induced apoptosis and took on arresting effect in G_0/G_1 phase in Hela cells by the concentration-dependent fashion.Conclusion:Cisplatin can induce the apoptosis and arrest cell cycle in G_0/G_1 stage in Hela cells,which may be one of important mechanisms of cisplatin against cervical carcinoma.