Studies on Technology for Compound Producing Bacillus natto Preparations by Solid State Fermentation

In order to determine the optimum fermentation conditions of bacterium in probiotics,the solid state fermentation conditions such as inoculum ratio,inoculum size,initial pH value,and initial moisture,fermentation temperature and time of Bacillus natto and Saccharomyces cereviae were optimized manuscript by L9(34)orthogonal experiment.The best results were achieved at the following conditions: initial pH value 6.5,initial moisture 70%,inoculum ratio1∶1,inoculum size 10%,fermentation temperature 34 ℃ fermentation time 5 days.The total number of Bacillus natto reached 1.74×1010,and the total number of Saccharomyces cereviae was 1.76×109after fermentation.