2007•Jiepou kexue jinzhanRequires access
Distribution of ZnT7 Protein and Free Zinc Ions in Mouse Ependyma and Choroid Epithelium
Wang Zhan-you
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Abstract
Objective To investigate the localization of zinc transporter 7(ZnT7) protein and free zinc ions in ependyma cells in mouse spinal cord and choroid plexus cells in mouse brain.Methods Zinc selenide autometallography(AMG) was used to detect free zinc ions in mouse ependyma and choroid plexus 1.5 hours after sodium selenite treatment;SABC immunohistochemistry was used to detect the location of ZnT7 protein in ependyma and choroid epithelium.Results One and half hours after selenite intraperitoneal injection,almost all ependyma and choroid plexus cells showed AMG positive reactivity.ZnT7-immunoreactive cells were observed in almost all ependyma cells and choroid plexus cells,and the distribution of the immunoreactivity was similar to the AMG staining.Conclusion The abundant distribution of both ZnT7 protein and free zinc ions in mouse ependyma and choroid plexus cells suggests that zinc may play a important role in ependyma and choroid plexus.