Research on Chemical Constituents of Ethyl Acetate Fraction from Li Nationality Medicine Elaeagnus gonyanthes Caulis et Folium

Objective:To study the chemical constituents of ethyl acetate fraction from Elaeagnus gonyanthes Caulis et Folium.Method: The medicinal plants were first extracted by 70% ethanol-water and then partitioned with petroleum ether,ethyl acetate and n-butanol,respectively.The chemical constituents of the ethyl acetate fraction were separated and purified by column chromatography methods and their structures were identified by physiochemical properties,thin-layer chromatography and spectra analyses.Result: 6 compounds were isolated and identified as β-sitosterol(1),lupeol(2),oleanolic acid(3),ursolic acid(4),α-amyrin(5),β-daucosterol(6),respectively.Conclusion: All the six compounds were isolated from this plant for the first time.