Study on genotyping of ABO blood group and its application in Chinese Han population.

Objective To study ABO gene polymorphism of Chinese Han population and apply ABO genotyping technology to solve the serological problems of clinical transfusion. Methods After DNA was extracted from peripheral blood by rapid salt method, we employed the polymerase chain reaction with sequence -specific primers (PCR-SSP) based on ABO genotyping using 7 designed primers to amplify ABO gene and the allele of difficult blood type after the serologic tests including absorption-elution tests, saliva neutralizing or inhibitor substances tests and others were used. Results We had detected alleles in a population of 260 Chinese Han individual. The gene frequencies of these alleles were 01 0.5827, B 0.1846,A101 (A467C)0.0096, A102/103(A467T) 0.2231 respectively. Amoung six individuals suspected as A2 by serological tests two were proved to be A20101, the rest were A102/10301. Three children of a family were para-Bombay types with ABO types A102B, A102B and A102O1 respectively. Conclusion ABO genotyping technique, much better than serologic tests, is a simple, rapid and accurate method for determing the difficult cases of ABO genotyping. Furthermore, we found some allele structure are different to that reported before.