2006•Chinese Journal of Nephrology,Dialysis & TransplantationRequires access
Effect of cordceps sinensis on expressions of TGF-β_1 and CTGF in Rats with diabetic nephropathy
Weisheng Peng
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Abstract
Objective:To observe the effects of cordceps sinensis (CS) on the expression of TGF-β_1 and CTGF in the renal tissue of diabetic nephropathy(DN) rats, and to investigate the anti-fibrosis mechanism of CS. Methodology: Fourty-five male Wistar rats were randomly divided into normal control group, DN group and treated group with CS 5.0 g/(kg·d ). At week 2, week 4 and week 6, five rats of three groups were sacrificed to test renal fibrotic injury, serum creatinine (SCr), and 24hr urinary protein count. The expression of TGF-β_1, CTGF and Collagen Ⅳ were examined by immunohistochemical technique and RT-PCR. Results:Compared with DN model group, the levels of SCr, 24h urinary protein excretion, and glomerular fibrosis was reduced significantly in CS treatment group (P0.05 or P0.01). At the same time, the expression of TGF-β, CTGF, Col Ⅳ was significantly decreased in CS treated group (P0.05 or P0.01). Conclusion:Cordceps sinensis may be play a role in renoprotection and anti-fibrosis in rats with DN, and it maybe contribute to reducing the expression of TGF-β_1 and CTGF.