Application of the antibody for frozen section to the praffin section

Aim:To explore how to make the antibody which is originally applied to the frozen section be used for the paraffin section through a series of treatments of the tissue antigen recovery. Methods:In addition of the routine immunohistochemistry, the tissue antigen was recovered through a series of treatments, including Triton X 100, incubation with 0.1 ～0.2 mol·L -1 glycine at 4 ℃overnight or under room temperature 2 h and microwave heat recovery in 0.01 mol·L -1 citrate buffer pH6.0 at 95～98 ℃ 10～20 min. The immunoreactivity (IR) of 3 kinds of antibodies for the frozen section was observed and compared with that on the paraffin section.Results: In 3 kinds of antibodies, the GDNF IR was much readily than the caspase 3 IR and XRCC1 IR. The IR of each antibody on the frozen section or smear specimens prevailed over that on the paraffin section. Conclusion: The IR on the paraffin section could be improved after treatment with a series of the tissue antigen recovery, and the effect may be concerned with different kinds of antibodies.