Influences of Combination Application of B_9 and 6-BA on the Fresh-keeping Effect of Cut Flowers of Gladiolus hybridus

[Objective] The research aimed to provide theoretical basis and technical guidance for improving the fresh-keeping effect on cut flowers of Gladiolus hybridus. [Method] With G. hybridus variety Fenxiu as tested materials, based on the observation on the indexes such as the life, the ornamental value, water balance value, the change rate of fresh weight, anthocyanin content and the relative permeability of petal plasma membrane, the effects of different combinations of B9 and 6- benzyl adenine(6-BA)on the fresh-keeping effect of cut flowers of G. hybridus were discussed. [Result] The vase life of cut flowers in each treatment were longer than that of CK. Compared with mother liquor, the vase life in the treatments of adding 6-BA and B9 were obviously prolonged. An obvious interaction accrued between 6-BA and B9 had an obvious interaction. Compared with B9, 6-BA had better effects on increasing the fresh weight of cut flowers of G. hybridus, improving the water conditions in the body of cut flowers, etc. Each treatment of different antistaling agents could all inhibit the production of membrane lipid peroxide malondialdehyde in plant body and maintain the stability of cell membrane structure. The anthocyanin content in each treatment showed a trend of first increasing and then decreasing with the increment of the vase days. [Conclusion] Considering synthetically, the fresh-keeping effect of 4 % sucrose +300 mg/L 8-hydroxyquinoline +150 mg/L boric acid +20 mg/L 6-BA +200 mg/L B9 was best and its vase life reached 22 d.