The Research on Tissue Culture and Rapid Propagation of Phalaenopsis Hybrid

The flower-stems of the plant were used as explants primarily induced to study the propagation pathway of Phalaenopsis Hybrid cultured in vitro,and the regeneration systems of the plant were constructed.The result indicated that the most numbers of the sprouts were observed by using MS medium supplemented with 6-BA 5 mg/L,NAA 0.3 mg/L,Sugar 30 g/L and Agar 6 g/L as primary culture medium;the optimal propagation medium for the highest numbers of leaf and propagation coefficient was MS medium supplemented with 6-BA 7 mg/L,NAA 1.0 mg/L,Sugar30 g/L,Agar 6 g/L and Coconut juice 200 mL/L;the optimal root medium was half of the modified KC medium supplemented with IBA 1 mg/L and NAA 0.5 mL/L.