Isolation and identification of chemical constituents from leaves of Crataegus pinnatifida Bge.

Objective To study the chemical constituents of the leaves of Crataegus pinnatifida Bge..Methods Seven compounds were isolated by chromatography on silica gel column,Sephadex LH-20,ODS chromatography column and HPLC,their structures were confirmed on the basis of physico-chemical constants and spectroscopic analysis.Results Seven compounds were isolated from 80% ethanol-water extraction and their structures were identified as α-tetrahydrobisabolen-2,5,6-triol(1),10,11-dihydroxynerolidol(2),3,5,4′-trimethoxy-4-hydroxyl-biphenyl(3),(+)-7R,8S-5-methoxy dihydrodehydroconiferyl alcohol(4),5,4′-dimethoxy-biphenyl-4-ol-3-O-β-D-glucoside(5),quercetin(6),vitexin(7).Conclusions Compound 1 is a new natural product and compounds 2-4 are isolated from Crataegus genus for the first time.